In a methane digester where fermentation can be stopped normally, the rate of methanogens producing methane depends on the rate of application of non-methanogens to organic synthesis. Shandong Biogas Purification Equipment Company reminds us that in order to further improve the production of biogas, it is not the continued enrichment of methanogenic bacteria, but the hydrolysis rate of non-methanogenic bacteria to organic matters, especially the synthesis rate of cellulose. Therefore, it is an important measure to improve the application rate of organic matter synthesis and biogas production to provide dominant microbial flora for biogas digesters and improve the metabolic function of non-methanogenic bacteria.
In order to improve the synthetic application rate and gas production of biogas digester fermentation raw materials, more methanogenic bacteria (anaerobic sludge) are attached great importance to in biogas digesters at home and abroad. In addition to the above measures, it is an important way to improve the gas production of biogas digesters by providing the dominant bacterial flora of hydrolytic bacteria, improving the metabolic function of non-methanogenic bacteria and making organic matter more thoroughly synthesized. It is particularly important to take this measure to dispose of biogas fermentation raw materials with high solid content and more difficult to synthesize organic matter.
Shandong Biogas Purification Equipment Co., Ltd. has found that in the anaerobic digestion process of pig manure and chicken manure, the activities of various hydrolytic enzymes (protease, cellulase, lipase and amylase, etc.) are proportional to the biogas production, and the activities of hydrolytic enzymes are near the peak value of biogas production, and the change curve of hydrolytic enzymes is similar to that of biogas production. This result has not been reported at home and abroad. It was also indicated that proper addition of hydrolytic enzymes could accelerate the degradation of cellulose in fermentation raw materials and significantly improve biogas production.
The natural straw is composed of cellulose, hemicellulose, lignin, local protein, amino acid, tannin and so on. Because of the complexity of natural lignocellulose structure, the difficulty of stopping bioconversion is how to efficiently degrade it into fermented sugar. Separation pretreatment is an effective step to improve the hydrolysis of cellulose by cellulase. The combination of cellulase production, cellulose hydrolysis and alcohol fermentation can improve the efficiency of bioconversion and reduce the cost of conversion. It is well known that there is a layer of wax on the epidermis of plant materials such as wheat straw, rice straw and corn straw. If it is not disposed of, it will go down into the pond. Water will not easily enter the straw through the waxy layer. Cellulose is difficult to decay and synthesize, and can not be used by methane bacteria, and it will form bubbles or crusts.
Because the degradation ability of lignocellulose by microorganisms is weak, the hydrolysis process of crop straw is slow and the level of hydrolysis is low, the anaerobic digestion time of crop straw is long, the digestibility is low, the gas production is low, and the input-output benefit is poor, so the wide application of straw in anaerobic fermentation is limited.
Therefore, how to effectively degrade lignocellulose in crop straw after pretreatment has become a key issue in straw biological disposal.
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